Additional file 1: of Deficiency of the BMP Type I receptor ALK3 partly protects mice from anemia of inflammation

Experimental design. (a) Mice were fed an iron deficient diet since weaning and throughout the experiment. At the age of 12 weeks, female Alk3fl/fl; Alb-Cre and Alk3fl/fl mice were intraperitoneally injected with 5 × 108 particles/mouse of heat-killed Brucella abortus (BA) or saline. Two weeks later blood and organs were collected. (b) 12 week old Alk3fl/fl; Alb-Cre and Alk3fl/fl female mice fed a regular diet were intravenously inoculated with 1 × 106 colony forming units (CFUs) of Staphylococcus aureus. Twenty-four hours later blood and organs were collected. (TIFF 164 kb)