Additional file 1: of Deferasirox drives ROS-mediated differentiation and induces interferon-stimulated gene expression in human healthy haematopoietic stem/progenitor cells and in leukemia cells

Figure S1. Qualitative and quantitative analysis of ROS induction in leukemia cells by confocal microscopy. A: Representative laser scanning confocal microscopy imaging of intracellular and mitochondrial ROS production in living cells treated with 100 μM DFX ± 10 mM NAC, assessed by DCF (upper panels) and Mitosox (lower panels) respectively. Magnification of selected areas (indicated by the white frame) is shown at the bottom of each panel. The images are representative of three different preparations of Kasumi-1 yielding similar results. The histograms on the right show the quantitative analysis of the DCF or Mitosox-related fluorescence/cell; the values are means ± SEM of three independent experiments under each condition wherein the digitalized fluorescence images from at least five randomly selected optical fields (each containing about 10 cells) were analysed (*p < 0.05 vs CTRL, #p < 0.05 vs DFX). B: Representative laser scanning confocal microscopy imaging of mitochondrial mass assessed by the specific Mitotracker-red probe in living Kasumi-1 cells treated with 100 μM DFX for 24 h. Magnification of selected areas (indicated by the white frame) is shown at the bottom of each panel. The histogram on the left shows the quantitative analysis of pixel intensity of probe-related fluorescence/cell; the values are means ± SEM, referring to at least ten optical fields randomly selected for each condition and clustered from three independent cell preparations (*p < 0.05 vs untreated cells (CTRL)). (TIF 10263 kb)