Additional file 1 of A novel family of expression vectors with multiple affinity tags for wheat germ cell-free protein expression

Additional file 1 : Supplementary Figure 1. Schematic illustrations of modified pEU3-NII plasmids. Utilized restriction endonucleases (the unique cutters are shown in bold), plasmid names and sizes are indicated on the maps. Ampicillin resistance gene (AmpR), origin of replication (ori), T7 promoter, glutathione-S-transferase (GST) gene, tobacco etch virus protease recognition site (TEV site with amino acid sequence E(N/D)LYFQS), LIC (ligation-independent cloning) site. Plasmid maps produced by using SnapGene software (from GSL Biotech; available at snapgene.com).