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Additional file 1: of A novel BMI-1 inhibitor QW24 for the treatment of stem-like colorectal cancer

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posted on 2019-10-23, 08:29 authored by Jinhua Wang, Yajing Xing, Yingying Wang, Yundong He, Liting Wang, Shihong Peng, Lianfang Yang, Jiuqing Xie, Xiaotao Li, Wenwei Qiu, Zhengfang Yi, Mingyao Liu
Figure S1. The screening process of compounds to down-regulate BMI-1. A, Schematic illustrates the screening process of QW24 which can down-regulate BMI-1. B and C, The compounds with IC50 less than 1 μM in HCT116 and HT29 cells were listed (B) and the chemical structures of the compounds were shown (C). D, HCT116 cells were treated with the indicated compounds and concentrations for 12 h. Cells were lysed and BMI-1 protein level was measured by western blotting analysis. E, HCT116 cells were treated with the indicated concentrations of QW24 or QW30 for 24 h. Cells were lysed and BMI-1 protein level was measured by western blotting analysis. F, The normal cell lines, including human normal liver cell L02, human skin fibroblast cell HAF, human normal colon epithelium cell NCM460 and human umbilical vein endothelial cell HUVEC, were seeded in 96-well plates (3000 cells/well) and treated with 0, 0.5, 1, 2, 4 μM of QW24 after cells were attached. After 72 h incubation, cell growth was measured by SRB assay. Data are presented as mean ± s.d. (n = 5); **, P < 0.01; ***, P < 0.001. (DOCX 100 kb)

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