Additional file 1: of A new role of hindbrain boundaries as pools of neural stem/progenitor cells regulated by Sox2

Confocal analysis of st.18 hindbrains. Confocal analysis if 18HH hindbrain stained with DAPI. (a–d) Sequential Z-stack from 0 to –12 μm of a boundary/rhombomere area. (e–f) Confocal-generated 3D model; higher magnification of boxed area in (e) is shown in (f). Hindbrain boundaries are denoted by dashed lines. r = rhombomere, b = boundary; VZ = ventricular zone; SVZ = sub-ventricular zone. (TIF 1891 kb)