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Additional file 1 of AKT2 deficiency impairs formation of the BCR signalosome

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posted on 2020-04-08, 09:05 authored by Zuochen Du, Di Yang, Yongjie Zhang, Xingtian Xuan, Han Li, Leling Hu, Changshun Ruan, Liling Li, Anwei Chen, Liang Deng, Yan Chen, Jingwen Xie, Lisa S. Westerberg, Lu Huang, Chaohong Liu
Additional file 1: Fig. S1. The effect of AKT2 deficiency on the germinal center (GC) B cells is cell-intrinsic. CD45.1 mice were irradiated with sublethal radiation (6Gy), and then intravenously injected with 1 × 107 total BM cells containing AKT2 deficient BM (CD45.2) and WT BM (CD45.1) at a ratio of 1:1. After 8 weeks, B cells from the chimera mice were labeled with specific antibodies for surface marker of FO (A), MZ (C) and GC (E) B cells, and analyzed using flow cytometry (n = 3). Shown are the frequency (+SEM) of different populations (B, D, F). T-test was used to do the statistics, *p < 0.01. Fig. S2. The gene expression of Btk, Was and Cd19 is comparable in splenic B cells from WT and AKT2 KO mice. The total RNA was isolated from the splenic B cells from WT and AKT KO mice (n = 3). The gene expression of Btk (A), Was (B) and Cd19 (C) were detected by real-time PCR, and Gapdh was used to standardized the mRNA expression. T-test was used to do the statistics.

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81701628 cstc2017jcyjAX0439

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