Additional file 1: of AKT1low quiescent cancer cells persist after neoadjuvant chemotherapy in triple negative breast cancer

S2 Antibody target specificity is unaffected by sequence of primary antibody application. Merged (right) and single color (left) confocal microscopy images at × 60 of an untreated primary TNBC tumor stained in an alternate sequence: pan-AKT ➔ H3K9me2 ➔ HES1 (c.f. standard sequence of H3K9me2 ➔ pan-AKT ➔ HES1) demonstrating consistent cytoplasmic pan-AKT (green) and HES1 (red) staining and nuclear H3K9me2 (yellow) staining in an example QCC (white arrows). (PDF 3624 kb)