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Additional file 1: Table S1. of Changes in hormone flux and signaling in white spruce (Picea glauca) seeds during the transition from dormancy to germination in response to temperature cues

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posted on 2015-12-18, 05:00 authored by Yang Liu, Kerstin Müller, Yousry El-Kassaby, Allison Kermode
Description of genes and primer pairs used for qPCR. Figure S1. Profiles of cytokinins and their metabolites in seeds of white spruce (as determined by UPLC/ESI-MS/MS) during moist-chilling at 3 °C (0, 10, and 21 d), and during germination (6, 24 and 80 h) and seedling growth (9 d). Each data point is the average of two biological replicates. cis-ZR, cis-Zeatin riboside; iPR, Isopentenyladenine roboside; cis-ZOG, cis-Zeatin-O-glucoside. Figure S2. Transcript levels of various marker genes at 6, 24, and 80 h following transfer of seeds to standard germination conditions (i.e. 8-h photoperiod and 30/20 °C) (black bars), constant darkness with a 30/20 °C cycle (light grey bars), or constant 3 °C with an 8-h photoperiod (dark grey bars). Each data point is the average of three biological replicates. Bars indicate the SEM. Figure S3. C(t) values for all three reference genes across studied time-points. Figure S4. Repeatability of hormone quantification analyses. Note: variation between two experimental replicates in four metabolites (IAA-Asp, IAA, PA, and ABA) was distinguished by colours in the panel. (DOCX 475 kb)

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