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Additional file 1: Table S1. of Antimicrobial activities of actinomycetes isolated from unexplored regions of Sundarbans mangrove ecosystem
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posted on 2015-08-21, 05:00 authored by Sohan Sengupta, Arnab Pramanik, Abhrajyoti Ghosh, Maitree BhattacharyyaDifferent culture media with supplemented NaCl salt concentration (w/v) for isolation of actinobacteria. Table S2. Sporechain characteristic of nine actinomycetes as studied by scanning electron microscopy and results of the biochemical tests. Table S3. Colony characteristics of the isolates in different media.G- Growth, A-Aerial mycelium, R-Reverse colour, SP-Soluble pigment. Table S4. Biolog assay for nine selected strains. 71 carbon sources and 23 chemical utilization characteristics were examined in this study. Presence of a positive reaction was assigned the binary value 1, and negative reaction was assigned a binary value of 0. For each of the biochemical characteristics, at least one strain differed in any one characteristic. Table S5. Fatty acid methyl ester (FAME) analysis (%) for nine actinomycetes. Table S6. Results of the identity analysis of isolated actinobacteria strains based on 16S rRNA gene sequence performed on the EzTaxon server (last accessed on 05/06/2015). Table S7. Antimicrobial activity Comparison between SMS_SU21 crude extract and the HPLC purified fraction SMS_SU21-C. Table S8. Characteristics of the peaks obtained by GC-MS analysis. Figure S1. Colony morphology of the nine selected strains on seven different medium: Right-aerial mycelium, Left-substrate mycelium. Figure S2. a) HPLC chromatograph of SMS-SU21-crude extract.SMS_SU21C is the bioactive peak. b) TLC profile of the active crude (Viewed under UV 254 nm). (DOCX 6739 kb)
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16 S rRNA gene sequencebioactive peak . b TLC profile71 carbon sourcesRight-aerial myceliumHPLCSMSR-Reverse colourFAMELeft-substrate myceliumNaCl salt concentrationculture mediaactinomyceteSMS-SUTable S 5. Fatty acid methyl esterA-Aerial myceliumDOCX 6739 kbTable S 3. Colony characteristicsAntimicrobial activitiesSP-Soluble pigment. bSundarbans mangrove ecosystemidentity analysisEzTaxon serverGC-MS analysisFigure S 2.scanning electron microscopyUV 254 nmFigure S 1. Colony morphology23 chemical utilization characteristicsTable S 4. Biolog assayTable S 2. Sporechainactinobacteria strainsTable S 6. Results
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