13045_2017_459_MOESM1_ESM.pdf (351.07 kB)
Additional file 1: Figure S1. of Vaccination with poly(IC:LC) and peptide-pulsed autologous dendritic cells in patients with pancreatic cancer
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posted on 2017-04-07, 05:00 authored by Shikhar Mehrotra, Carolyn Britten, Steve Chin, Elizabeth Garrett-Mayer, Colleen Cloud, Mingli Li, Gina Scurti, Mohamed Salem, Michelle Nelson, Melanie Thomas, Chrystal Paulos, Andres Salazar, Michael Nishimura, Mark Rubinstein, Zihai Li, David ColeCytokine secretion by DCs. Frozen DCs obtained from patients were thawed and cultured with GM-CSF/IL-4 overnight before adding poly (IC:LC) for maturation overnight. The supernatant was collected after 16Â hrs. and evaluated for cytokines IL12 (upper panel), and IL10 (lower panel) as per the manufacturerâs protocol. Figure S2. PFS and OS details. The raw data showing details of progression free survival (PFS) and the median overall survival (OS) is presented in tabular form. Figure S3. Gating scheme for flow cytometry analysis. PBMC was obtained from patients post vaccination and cryopreserved cells were stained using the multiple fluorochrome-conjugated antibodies. Cells were gated based on singlets (FSC-A vs FSC-H), size (SSC vs FSC-H), a live-dead stain (L/D), and subsequently markers to determine specific cell phenotypes. A) CD3+ T cells were phenotyped for CD4 and CD8. B) CD19 B cells were identified. C) NK cells were identified based on their CD56 and CD16 expression. The data was acquired using BD FACS Aria and analyzed using FlowJo software. (PDF 351Â kb)
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CD 19 B cellscytokines IL 12tabular formcryopreserved cellsFSC-AFSC-HNK cellspancreatic cancer Cytokine secretionpatients post vaccinationGM-CSFCD 8. BOS detailsCD 4Figure S 2. PFSSSCPBMCcell phenotypeslive-dead stainFlowJo softwareCD 16 expressionflow cytometry analysisCD 56DCpeptide-pulsed autologous dendritic cells16Â hrsIL 10PDF 351Â kbBD FACS Ariafluorochrome-conjugated antibodiesICFigure S 3. Gating scheme
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