Additional file 1: Figure S1. of Sublethal dose of irradiation enhances invasion of malignant glioma cells through p53-MMP 2 pathway in U87MG mouse brain tumor model

The illustration of the experimental process to assess radiation effect on brain tumor in vivo. After implantation U87-Fluc cells into brain, tumor bioluminescence of mouse brain bearing U87-Fluc was continuously observed from day 10 to 28 using the IVIS 100 imaging system. Radiation was exposed to either Single dose 6Gy or 2Gy fractions 5 times for 7 days on 14 day after U87-Fluc implantation. Mice brain were performed with Hematoxylin and eosin or immunohistochemical staining after execution on 30 day. (PPTX 381 kb)