Additional file 1: Figure S1. of Stress induced nuclear granules form in response to accumulation of misfolded proteins in Caenorhabditis elegans

High sucrose also induces SINGs. Young adult worms expressing GFP::Ub were soaked in either M9, 500 mM NaCl, or 815 mM sucrose for 1 h. Worms were then imaged on the confocal microscope and assessed for the presence of SINGs in oocyte nuclei. A higher concentration of sucrose was used due to the lower osmolarity of sucrose as compared to NaCl. For each condition, a total of 900 oocytes were observed from 3 independent experiments (n = 30 worms). Statistical significance was calculated by a Fisher’s Exact test: ***p < 0.001. (JPG 234 kb)