13024_2017_169_MOESM1_ESM.tif (1.46 MB)
Additional file 1: Figure S1. of Soluble oligomeric amyloid-β induces calcium dyshomeostasis that precedes synapse loss in the living mouse brain
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posted on 2017-03-21, 05:00 authored by Michal Arbel-Ornath, Eloise Hudry, Josiah Boivin, Tadafumi Hashimoto, Shuko Takeda, Kishore Kuchibhotla, Steven Hou, Carli Lattarulo, Arianna Belcher, Naomi Shakerdge, Pariss Trujillo, Alona Muzikansky, Rebecca Betensky, Bradley Hyman, Brian BacskaiBiochemical characterization of Aβ oligomers present in Tg conditioned media (TgCM). a Representative profile showing the amount of Aβ40 detected by ELISA in each fraction after separation of the different oligomeric species present in TgCM (red bars) by size-exclusion chromatography (SEC, Superdex 75 SEC columns). The molecular weight markers (kDa) that ran at the same conditions are indicated above (arrowheads). b Measurements of oligomeric Aβ within each SEC fraction show that most of oAβ is of low molecular weight (fractions 12–18).. A smaller peak of high-molecular weight species was also detected in TgCM (fractions 2–6 in both ELISAs). (TIF 1492 kb)
