Additional file 1: Figure S1. of Soluble oligomeric amyloid-β induces calcium dyshomeostasis that precedes synapse loss in the living mouse brain

Biochemical characterization of Aβ oligomers present in Tg conditioned media (TgCM). a Representative profile showing the amount of Aβ40 detected by ELISA in each fraction after separation of the different oligomeric species present in TgCM (red bars) by size-exclusion chromatography (SEC, Superdex 75 SEC columns). The molecular weight markers (kDa) that ran at the same conditions are indicated above (arrowheads). b Measurements of oligomeric Aβ within each SEC fraction show that most of oAβ is of low molecular weight (fractions 12–18).. A smaller peak of high-molecular weight species was also detected in TgCM (fractions 2–6 in both ELISAs). (TIF 1492 kb)