12896_2017_363_MOESM1_ESM.pdf (46.14 kB)
Additional file 1: Figure S1. of Pollen-mediated gene flow from transgenic to non-transgenic switchgrass (Panicum virgatum L.) in the field
journal contribution
posted on 2017-05-02, 05:00 authored by Reginald Millwood, Madhugiri Nageswara-Rao, Rongjian Ye, Ellie Terry-Emert, Chelsea Johnson, Micaha Hanson, Jason Burris, Charles Kwit, C. StewartSouthern blot analysis of T0 transgenic switchgrass events containing stable integration of the T-DNA. Blot was probed with 32P-labeled pporRFP gene fragment. Lanes labeled with ‘2BR’ contain transgenic line genomic DNA samples, lane ‘ST1 control” is the nontransgenic control genomic DNA sample, lane ‘2BR plasmid’ contains the plasmid positive control. Genomic DNA was digested BamHI genomic DNA (10 μg) from each plant sample. (PDF 46 kb)