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Additional file 1: Figure S1. of Metabolic determinants of the immune modulatory function of neural stem cells
figure
posted on 2016-09-02, 05:00 authored by Denise Drago, Veronica Basso, Edoardo Gaude, Giulio Volpe, Luca Peruzzotti-Jametti, Angela Bachi, Giovanna Musco, Annapaola Andolfo, Christian Frezza, Anna Mondino, Stefano PluchinoNSCs induce downregulation of the activation cell surface marker CD44 on purified CD4+ T cells. CD4+ T cells were purified by negative selection from total LNC, labelled with the vital dye CFSE, and cultured on CD3/CD28 coated mAb in the absence or the presence of NSCs, Th1 NSCs or Th2 NSCs for 72 h. Cells were surface stained with anti-CD4 and CD44 antibodies. Absolute fractions of CFSEdimCD44low CD4+ T cells are depicted. Data are expressed as mean % (±SD) from n ≥ 3 independent experiments. *p ≤ 0.05 and **p ≤ 0.01 vs. T cells.
