Additional file 1: Figure S1. of Ligation-free ribosome profiling of cell type-specific translation in the brain

Sensitivity of conventional and ligation-free strategies. Ligation-free and conventional libraries were generated from a serially diluted 34-base RNA oligonucleotide and analyzed via Bioanalyzer following an equal number of PCR cycles for each library. All ligation-free library preparations except for the 0.01 ng sample were loaded onto the Bioanalyzer at a 1:10 dilution to avoid saturating the detector at high concentrations. Detectable libraries were successfully generated for all concentrations using the ligation-free method but could only be generated using conventional methods for the 100- and 10-ng inputs. (PDF 665 kb)