Additional file 1: Figure S1. of Inhibition of hypothalamic leukemia inhibitory factor exacerbates diet-induced obesity phenotype

The anatomical and cellular distribution of LIF and LIF receptor in the hypothalamus of mice. Hypothalamic 5.0 μm frozen sections were prepared from 5-week old mice fed on chow. Immunofluorescence staining was performed using antibodies against LIF (A), LIF receptor (LIFR) (B), alpha-MSH (aMSH) (A, B). The high magnification images depict details of cells (arrows). Nuclei was labeled using DAPI. Color code and magnifications are presented in the panels. Figures are representative of three independent experiments. Figure S2. The impact of LIF immunoneutralization on STAT3 phosphorylation in the hippocampus and hypothalamus. The expression of phosphor-STAT3 (pSTAT3) protein was evaluated by immunoblot in the hippocampus (A) and hypothalamus (B) of obesity-resistant mice treated with non-immune IgG or anti-LIF antibody for 15 days. N = 3–4; *p < 0.05 vs. IgG. Table S1. Composition of the diets. Table S2. Transcripts evaluated with the real-time PCR array. (PDF 18097 kb)