Additional file 1: Figure S1. of Inhibition of MMP-2 and MMP-9 decreases cellular migration, and angiogenesis in in vitro models of retinoblastoma

Inhibition of MMP-2 or MMP-9 reduced Rb viability and cell cycle progression. a, Y79 cells were cultured in the presence or absence of the MMPI overnight. Next day, we collected cells and assessed viability by Trypan Blue exclusion. Chemical inhibition of Y79 with MMPI significantly reduced cell yield when compared to untreated cells. b, RNA interference was used to confirm on-target effects of MMPIs. Y79 were cultured in the presence of either MMP2 or MMP9 siRNA. MMP2 and MMP9 knockdown groups showed significant reduction in cell yield, illustrating an on-target effect of MMPI. c, Imaging flow cytometry analysis showed inhibition of MMP9 prevents progression of Rb cell division using nuclear DRAQ5™ labeling. Bar graphs indicate results ± SEM to control. **p < 0.005. (TIF 434 kb)