Additional file 1: Figure S1. of Improvement of alkalophilicity of an alkaline xylanase Xyn11A-LC from Bacillus sp. SN5 by random mutation and Glu135 saturation mutagenesis

Bai, Wenqin; Cao, Yufan; Liu, Jun; Wang, Qinhong; Jia, Zhenhu

doi:10.6084/m9.figshare.c.3627413_D1.v1

12896_2016_310_MOESM1_ESM.doc (325.5 kB)

Additional file 1: Figure S1. of Improvement of alkalophilicity of an alkaline xylanase Xyn11A-LC from Bacillus sp. SN5 by random mutation and Glu135 saturation mutagenesis

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posted on 2016-11-08, 05:00 authored by Wenqin Bai, Yufan Cao, Jun Liu, Qinhong Wang, Zhenhu Jia

Schematic diagram of a high-throughput screening of the positive mutant. Table S1. Primers used for random mutagenesis, site-directed mutagenesis and site saturation mutagenesis. (DOC 325 kb)

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the Natural Science Foundation of China

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Xylanase Alkalophilicity Error-prone PCR Site-directed mutation Site-saturation mutagenesis Catalytic efficiency

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Additional file 1: Figure S1. of Improvement of alkalophilicity of an alkaline xylanase Xyn11A-LC from Bacillus sp. SN5 by random mutation and Glu135 saturation mutagenesis

Funding

the Natural Science Foundation of China

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