Additional file 1: Figure S1. of Grape seed-derived procyanidins alleviate gout pain via NLRP3 inflammasome suppression

The Raw 264.7 cells were treated with or without LPS (1 μg/ml) for 6 h and then stimulated with MSU crystals for another 6 h. Figure S2. The levels of ROS were assessed by calculating the ratio of positive-staining cells among 10,000 cells using flow cytometry. Raw 264.7 cells were treated with procyanidins (10 μM) for 20 min and then stimulated by MSU crystals for 3 h. (DOCX 698 kb)