Additional file 1: Figure S1. of Expression of von Hippel–Lindau tumor suppressor protein (pVHL) characteristic of tongue cancer and proliferative lesions in tongue epithelium

Staining of pVHL in clear cell renal cell carcinoma (RCC). (A) Staining of pVHL in normal renal tissues subjected to antigen retrieval. (a) Hematoxylin and eosin (HE) staining, (b–e) pVHL staining, (b) without antigen retrieval, (c) trypsinization, (d) microwaving treatment, (e) heating in an autoclave (arrow indicates proximal tubules), (f) heating in an autoclave (negative control staining with an unrelated monoclonal antibody). Bar indicates 50 μm. (B) Immunohistochemical staining of pVHL in clear cell RCC. (a) HE staining and (b) pVHL staining in clear cell RCC. Bar indicates 50 μm. (PDF 275 kb)