Additional file 1: Figure S1. of Endothelium-derived microparticles from chronically thromboembolic pulmonary hypertensive patients facilitate endothelial angiogenesis

Endothelial tube formation induced by CTEPH microparticles. HPAECs were cultured on Matrigel in serum-reduced (0.5 % serum) medium with different numbers of CTEPH microparticles, as indicated. Tube formation was scored after 18 h of incubation. **P < 0.01, comparison with untreated controls. Figure S2. Tube formation in HPAECs incubated with TGF-β and MPs. Representative images of tube formation in HPAECs incubated with MP fractions (full and endoglin−) from healthy and CTEPH plasma, with or without TGF-β (10 ng/mL;18 h). Bar = 50 μm. Figure S3. Endoglin levels in full MP fractions (containing endoglin+ MPs) and endoglin-depleted (endoglin−) MP fractions. Endoglin+ MPs were removed by Dynabeads-mediated immunoprecipitation and endoglin levels were measured with Human Endoglin/CD105 Quantikine ELISA Kit.***P < 0.001, Student t-test, n = 6. Figure S4. Pro-angiogenic factors in CTEPH EMP fraction. (a) Proteome Profiler™ Human Angiogenesis Array membrane was incubated with CTEPH EMPs (image representative of n = 2). (R) shows reference spots, (1) corresponds to Serpin E1 and (2) corresponds to uPA, as indicated. Full information about the microarray layout can be found on https://resources.rndsystems.com/pdfs/datasheets/ary007.pdf . A schematic diagram of microarray is shown in (b). (DOCX 479 kb)