13287_2015_260_MOESM1_ESM.pdf (16.75 MB)
Additional file 1: Figure S1. of Efficient differentiation of human embryonic stem cells to arterial and venous endothelial cells under feeder- and serum-free conditions
journal contribution
posted on 2015-12-30, 05:00 authored by Gopu Sriram, Jia Tan, Intekhab Islam, Abdul Rufaihah, Tong CaoImmunocytochemical analysis of pluripotency status of hESCs cultured over Matrigel. Figure S2. Immunocytochemical analysis of pluripotency status of hESCs cultured over fibronectin. Figure S3. Time-course analysis of early differentiation of hESCs towards mesodermal lineage. Figure S4. Time-course analysis of early differentiation of hESCs towards endodermal lineage. Figure S5. Differentiation of H9-hESCs towards endothelial lineage. Figure S6. Effect of VEGF on hESC-derived endothelial cells under serum-free conditions. Figure S7. Survey of angiocrine secretory profile of arterial and venous ECs using angiogenesis antibody array. Table S1: Legend of coordinates for the human angiogenesis array. Table S2. Sequences of primers used for real-time RT-PCR. Table S3. List of antibodies used for flow cytometry. Table S4. List of antibodies used for immunocytochemistry. Supplementary Methods. (PDF 17154 kb)