Additional file 1: Figure S1. of Charged residues next to transmembrane regions revisited: “Positive-inside rule” is complemented by the “negative inside depletion/outside enrichment rule”

The net charge per TMH plotted at each position; the positive-inside rule is stronger in TMHs from single-pass proteins than TMHs from multi-pass proteins. The net charge was calculated at each position as described in the Methods section for the (A) UniHuman and (B) ExpAll datasets. Net charge for TMHs from multi-pass proteins is shown in black, and the profile of TMHs from single-pass proteins is drawn in blue. (PDF 17 kb)