Additional file 1: Figure S1. of CD4+ and CD8+ T cells play a central role in a HDM driven model of allergic asthma

Investigation of mechanism driving acute inflammatory response to HDM challenge. Naïve male C57bl/6, TLR2, TLR4, Dectin-1 and Dectin-2 KO mice were anaesthetised (4 % isoflurane in oxygen for 3 min) and challenged with i.t. vehicle (saline) or HDM (25 μg/mice in 50 μl saline). 48 h after challenge the lungs were lavaged, lung weight (A) determined, BALF (B) and lung tissue (C) total white blood cell numbers (D) were determined. Data (n = 7–8) expressed as means ± S.E.M. *p < 0.05 vs saline challenged controls (Mann-Whitney test). #p < 0.05 vs. challenged WT controls, Mann-Whitney U-test. (DOC 185 kb)