Additional file 1: Figure S1. of Atypical Creutzfeldt-Jakob disease with PrP-amyloid plaques in white matter: molecular characterization and transmission to bank voles show the M1 strain signature

Western blot analysis of np-CJDMM1 and p-CJDMM1 (case #1) subcortical white matter. FC: frontal cortex; PC: parietal cortex. (a) Electrophoretic mobility of PK-digested PrPSc (i.e. PrP27–30) after separation in a 7 cm long gel. Blot was probed with the primary antibody 3F4. (b) CTF13 analysis after PrP deglycosylation with PNGase F. Blot was probed with the primary antibody SAF60. Relative molecular masses are expressed in kDa. Percentages (mean ± standard deviation) of CTF13 are referred to the total PrPSc amount: np-CJDMM1 = 12.8 ± 5.0, p-CJDMM1 = 14.1 ± 2.9. (TIFF 824 kb)