13287_2016_356_MOESM1_ESM.tif (5.7 MB)
Additional file 1: Figure S1. of Adipose tissue-derived stem cells suppress hypertrophic scar fibrosis via the p38/MAPK signaling pathway
figure
posted on 2016-08-02, 05:00 authored by Yan Li, Wei Zhang, Jianxin Gao, Jiaqi Liu, Hongtao Wang, Jun Li, Xuekang Yang, Ting He, Hao Guan, Zhao Zheng, Shichao Han, Maolong Dong, Juntao Han, Jihong Shi, Dahai HuCharacterization of mouse adipose-derived mesenchymal stem cells (mADSCs) in vitro. (A) Isolated mADSCs presented a short fusiform morphology under light field microscope. (B) Flow cytometry results show that mADSCs were positive for the mesenchymal stem cell markers CD29 (99.09 %), CD44 (97.17 %), and CD106 (65.55 %), but were negative for the hematopoietic stem cell markers CD31 (1.06 %) and CD45 (5.26 %). (C–D) Oil Red O and Alizarin Red S staining were used to confirm adipogenic and osteogenic differentiations, respectively. Scale bar = 50 μm. (TIF 5839 kb)