Additional file 1: Figure S1. of Adiponectin aggravates bone erosion by promoting osteopontin production in synovial tissue of rheumatoid arthritis

RAW264.7 migration assay by crystal violet staining. OASFs or RASFs were incubated with or without AD (1 μg/mL) in 24-well flat-bottom plates for 72 h. RAW264.7 cells were added to Transwell inserts and co-cultured with the above OASFs or RASFs for 24 h. The effect of RAW264.7 migration was measured (n = 3). Bars show the mean ± SD; *p < 0.05 vs control. The experiment was repeated three times, and representative pictures are shown. (PDF 459 kb)