Additional file 1: Figure S1. of Activation of human B cells negatively regulates TGF-β1 production

B cell activation reduced TGF-β1 mRNA expression. a A representative gating of naïve and memory B cells in PBMCs is shown. Cells were first gated for singlets (FSC-H vs. FSC-A) and lymphocytes (SSC-A vs. FSC-A), followed by a live/dead gate. Naïve B cells (CD27–) and resting and activated memory B cells (CD27+) were distinguished by CD19 versus CD27 gating. FSC-H, forward scatter height; FSC-A, forward scatter area; SSC-A, side scatter area. b Scatter plot indicates mean (± SEM) percentages of naïve (CD27–) versus memory (CD27+) B cell subpopulations (n = 6). c Quantitative RT-PCR analysis of human B cells for the expression of TGF-β1. Expression levels are normalized using the ΔΔCt method, relative to β-actin. Results show mean of 6 independent sets of samples. Errors bars represent SEM. (DOCX 286 kb)