12974_2015_465_MOESM1_ESM.pdf (106.21 kB)
Additional file 1: Figure S1. of Acid-sensing ion channel 1a contributes to the effect of extracellular acidosis on NLRP1 inflammasome activation in cortical neurons
journal contribution
posted on 2015-12-30, 05:00 authored by Yu-Chan Wang, Wei-Zu Li, Yu Wu, Yan-Yan Yin, Liu-Yi Dong, Zhi-Wu Chen, Wen-Ning WuIdentification of specificity of ASICs antibodies.Detection of ASIC protein in cortical neurons by double-staining immunofluorescence (original magnification Ă400). Nuclei were counterstained with Hoechst33258 (blue). ASICs were labeled with FITC (green). ASIC1, ASIC2, and ASIC3 protein cannot be detected after pretreatment with the corresponding antigen (Ag) peptide. (PDF 106 kb)