Additional file 1: Figure S1. of Acid-sensing ion channel 1a contributes to the effect of extracellular acidosis on NLRP1 inflammasome activation in cortical neurons

Identification of specificity of ASICs antibodies.Detection of ASIC protein in cortical neurons by double-staining immunofluorescence (original magnification ×400). Nuclei were counterstained with Hoechst33258 (blue). ASICs were labeled with FITC (green). ASIC1, ASIC2, and ASIC3 protein cannot be detected after pretreatment with the corresponding antigen (Ag) peptide. (PDF 106 kb)