Additional file 1: Figure S1. of A temporal and spatial contribution of asparaginase to asparagine catabolism during development of rice grains

Quantitative real-time PCR detection of transcripts for OsASNase1 (filled column) and OsASNase2 (opened column) in roots, leaf blades and leaf sheaths at different leaf positions in rice. Rice plants were grown hydroponically for 18 d in water and each organ was harvested individually; whole roots, 2nd leaf blade, 3rd leaf blade, 3rd leaf sheath, and 4th expanding leaf blade. Quantitative real-time PCR analyses were performed using gene-specific primers for OsASNase1, OsASNase2 and actin, respectively, as described in Table 1. The relative contents of these transcripts were normalized against actin transcript. Means of four independent samples and standard error values (n = 4) are indicated. Significant differences between OsASNase1 and OsASNase2 were identified by Student’s t-test, and are marked with asterisks: *P < 0.05 (PDF 401 kb)