Additional file 1: Figure S1. of 6-Mercaptopurine attenuates tumor necrosis factor-α production in microglia through Nur77-mediated transrepression and PI3K/Akt/mTOR signaling-mediated translational regulation

LPS-induced activation of PKA and MSK1 are not suppressed by 6-MP. BV-2 cells were stimulated with 100 ng/ml LPS for the indicated times with or without 16 h pretreatment with 6-MP (50 μM). Immunoblots were set up to detect phosphorylated VASP (Ser157) and MSK1 (Ser376). Images are representative of three independent experiments. (TIF 400 kb)