Additional file 1: Fig. S1. of Zinc finger nuclease-based double-strand breaks attenuate malaria parasites and reveal rare microhomology-mediated end joining

Integration PCRs of parasites used in this study. a Integration of SpZFN and LsZFN. PCR with P53/P54 amplifies the whole Chr12 locus, resulting in 1448 bp in WT, 7850 bp for SpZFN and 7141 bp for LsZFN. The 5’ integration was amplified with P53/P12 or P53/P24, resulting in 1226 bp and 1610 bp for SpZFN and LsZFN, respectively. The 3’ integration was performed with P54/P55, resulting in 1181 bp. b Integration of Sp2ZFN and Ls2ZFN. PCR of the whole locus resulted in 6758 bp or 7145 bp for Sp2ZFN and Ls2ZFN, respectively. The 5’ integration was performed with P53/P56 or P53/P24, resulting in 1226 bp or 1610 bp for Sp2ZFN and Ls2ZFN. The 3’ integration was verified with P54/P10 (1982 bp). c Integration of TrapZFN and Uis4ZFN. PCR of the whole locus resulted in 6988 bp and 6970 bp for TrapZFN and Uis4ZFN, respectively. The 5’ integration was performed with P53/P22 or P53/P38, resulting in 1462 bp and 1504 bp for TrapZFN and Uis4ZFN, respectively. The 3’ integration was verified with P54/P8 (2588 bp). (PDF 1088 kb)