Additional file 16: of Integrated analysis identifies microRNA-195 as a suppressor of Hippo-YAP pathway in colorectal cancer

Figure S5. Silence of YAP1 expression inhibits colon cancer cell growth, invasion, migration and rescue assay. A. CCK8 assays of DLD1 and HCT116 cells after transfected (un-transfected) with siRNA YAP1. B-C. Shown are representative photomicrographs of colony formation assay after transfected with (without) siRNA YAP1 for eight days. D. Shown are representative photomicrographs of transwell invasion assay after transfected with (without) siRNA YAP1. E. Shown are representative photomicrographs of transwell migration assay after transfected with (without) siRNA YAP1. F. Expression of YAP1 mRNA in siRNA YAP1 treated and blank DLD1 cell. G. Western blot of YAP, TAZ, E-cadherin, Vimentin, ZEB2 protein in siRNA YAP1 treated and blank DLD1 cell. Assays were performed in triplicate. Means ± SD are shown. Statistical analysis was conducted using student’s t-test. *P < 0.05. **P < 0.01. ***P < 0.001. (TIF 3081 kb)