Additional file 16: of Hypoxia-induced Slug SUMOylation enhances lung cancer metastasis

Figure S16. The hypoxia-induced enhancement of Slug SUMOylation was associated with PP2A activity. HEK293T cells were transiently transfected with 3xFlag-tagged Slug and GFP-tagged SUMO-1 and then treated with a 1-μM dose of the PP2A inhibitor okadaic acid for 1 h. After washing out the inhibitor, the cells were exposed to hypoxia (1% O2 for 4 h). The lysates were analyzed by immunoblotting with the indicated antibodies. The expression levels of HIF1α were used to confirm that the cells were exposed to hypoxia. β-actin was used as the internal control. The asterisk and arrowhead indicate Slug modified and not modified by SUMO-1, respectively. (PDF 37 kb)