Additional file 15: Figure S13. of Systemic inflammation disrupts oligodendrocyte gap junctions and induces ER stress in a model of CNS manifestations of X-linked Charcot-Marie-Tooth disease

Disruption of astrocyte and oligodendrocyte GJs in inflamed cerebellum. a-f: Fixed coronal cerebellar cortex sections including white matter (WM) surrounded by the granule cell layer (GCL) show double immunostaining with Cx43 (green), Cx47 (red) and nuclear DAPI staining (blue). Immunoreactivity of both Cx43 and Cx47 is reduced in LPS treated mice of all genotypes (b, d, f) compared to their saline controls (a, c, e) as indicated. Insets showing higher magnification of individual oligodendrocytes show reduction of GJ plaque formation by Cx43 and Cx47 at the cell bodies and proximal processes of oligodendrocytes with a weak diffuse cytoplasmic Cx47 immunoreactivity indicating intracellular diffusion (f). Scale bar: 50 Οm. (TIF 21393 kb)