13024_2020_365_MOESM14_ESM.pdf (319.41 kB)
Additional file 14 of Dipeptide repeat proteins inhibit homology-directed DNA double strand break repair in C9ORF72 ALS/FTD
journal contribution
posted on 2020-02-25, 05:00 authored by Nadja Andrade, Melina Ramic, Rustam Esanov, Wenjun Liu, Mathew Rybin, Gabriel Gaidosh, Abbas Abdallah, Samuel Del’Olio, Tyler Huff, Nancy Chee, Sadhana Anatha, Tania Gendron, Claes Wahlestedt, Yanbin Zhang, Michael Benatar, Christian Mueller, Zane ZeierAdditional file 14. Model of DPR-mediated inhibition of DNA DSB repair. In nucleoli, PR binds and stabilizes NPM1 pentamers by binding to the acidic loop of NPM1 in a similar way that endogenous arginine-rich proteins like ARF bind and stabilize NPM1. Since NPM1 facilitates DNA DSB repair in the nucleoplasm as a monomer, we hypothesize that PR inhibits DNA DSB repair, in part, by preventing the translocation of NPM1 from the nucleolus to the nucleoplasm.