Additional file 13: of Dual species transcript profiling during the interaction between banana (Musa acuminata) and the fungal pathogen Fusarium oxysporum f. sp. cubense

Figure S6. Verification of the mutant (ccp1) and the complemented (Comp) Foc strains. (A) Green fluorescence picture of mycelium from the mutant Foc strains (ccp1). (B) PCR amplification of the HYG + GFP cassette from the ccp1 strains using primer pairs H1/H2. Lane 1 to 6: the ccp1 strains and the fragments of about 490 bp were amplified; lane 7: wild type Foc; lane 8: the positive control (pCT74 vector as template); lane 9: the negative control (no template). (C) PCR amplification of CCP1 gene from the ccp1 strains using primer pairs F/R. Lane 1 to 6: the ccp1 strains. The CCP1 gene was completely knocked out in the ccp1 strains of lane 2, 4 and 6. Lane 7: the wild type Foc TR4. Lane 8: The negative control (no template). (D) Verification of the inserted 5′- and 3′- flanking sequences in the ccp1 strains. Lanes 1 to 6: The 5′-flanking fragments (A) (about 2.0 kb) PCR product using primer pairs F1/MF2 and the ccp1 strains as templates. Lane 7: wild type Foc DNA as template. Lane 8: the negative control (no template). Lanes 9 to 14: The 3′-flanking fragments (B) (about 1.9 kb) PCR product using primer pairs MF3/F4 and the ccp1 strains as templates; Lane 15: the wild type Foc DNA as template; Lane 16: the negative control. (E) Verification of the Comp strains. The PCR products using the primer pairs F/R. Lane 1 to 5: the Comp strains 1 to 5 as templates. Lane 6: the negative control with the ccp1 strain 6 as template. Lane 7: the positive control with the wild type as template. (TIF 14632 kb)