40168_2020_790_MOESM13_ESM.pdf (572.96 kB)
Additional file 13 of A pipeline for targeted metagenomics of environmental bacteria
journal contribution
posted on 2020-03-24, 12:58 authored by Anissa Grieb, Robert M. Bowers, Monike Oggerin, Danielle Goudeau, Janey Lee, Rex R. Malmstrom, Tanja Woyke, Bernhard M. FuchsAdditional file 13: Figure S13. Flow cytometric sort gates of unfixed samples, targeted with A: CF319a (Bacteroidetes) probe and Non338 (not binding) probe and B: Targeted with the probe mix Vis6-814/871 (Vis6-clade) and Non338 probe. The blue fluorescence (355 nm laser, 450/60 nm detector) shows all bacteria stained with DAPI. The percentage indicates the number of detected events within the sort gate. The amount of signals with green fluorescence (488 nm laser, 530/40 nm detector) increase with samples targeted with a probe compared to the Non338 probe.