Additional file 13: Figure S7. of Cleavage modification did not alter blastomere fates during bryozoan evolution

M. membranacea embryos treated with the MEK inhibitor U0126 (10 μM) from different developmental stages. All treatments developed at 10 °C and were fixed at 72 hpa. Arrowhead indicates when the U0126 treatments began for each experimental condition, represented by the horizontal colored lines. Representative phenotypes are shown for the 4, 8, and 18 hpa treatments. We scored 100 embryos under light microscopy for each treatment to obtain the ratio of severe/mild phenotypes. Treatments showing the severe phenotype are shown in orange, with the percentage of severe phenotypes indicated at the right end. Treatments without severe phenotypes were colored in yellow. (PNG 865 kb)