Additional file 12: of SUMO1 modification of KHSRP regulates tumorigenesis by preventing the TL-G-Rich miRNA biogenesis

Fig. S8. Expression of Flag-KHSRPΔN and Flag-SUMO1-KHSRPΔN in HeLa cells. HeLa cells were transfected with Flag-KHSRPΔN and Flag-SUMO1-KHSRPΔN. 48 h after transfection, 1/10 HeLa cells were harvested with SDS buffer for Input and 9/10 HeLa cells were harvested with the nuclear/cytosol fractionation kit. The expression of Flag-KHSRPΔN or Flag-SUMO1-KHSRPΔN was determined by Western blotting (PDF 333 kb)