Additional file 12: of Dual species transcript profiling during the interaction between banana (Musa acuminata) and the fungal pathogen Fusarium oxysporum f. sp. cubense

Figure S5. Construction of the mutant (ccp1) and the complemented (Comp) Foc strains. The capital A and B denote the 5′- and 3′-flanking sequences of CCP1 gene. Construction of the mutant cassette and its homologous recombination into the genome of Foc TR4 (upper). The fragments A and B were amplified with primers pairs F1/F2 and F3/F4, respectively, from Foc genomic DNA. The HYG + GFP cassette, including the HYG ORF, the TrpC promoter for HYG, the GFP ORF and the ToxA promoter for GFP, was amplified using M1/M2 from the vector pCT74. Construction of the complementation cassette and its homologous recombination into the genome of the ccp1 mutant strains (lower). The fragments A + CCP1 and B were amplified with primer pairs F1/CR and CF3/F4, respectively, from wild Foc TR4 genomic DNA. The NEO cassette, including the neomycin (NEO) ORF and its promoter, was amplified using C1/C2 from the vector pKOV21. Other primers labeled in this figure were used for the verification of mutant and complemented Foc strains in Additional file 13: Figure S6. (TIF 1019 kb)