Additional file 12 of Bivalent promoter hypermethylation in cancer is linked to the H327me3/H3K4me3 ratio in embryonic stem cells

Figure S10. Quintile chromatin configuration at human bivalent promoters. Left panel: As an alternative to k-means clustering we divided the data underlying Fig. S8 into H3K27me3:H3K4me3 quintiles determined by the ChIPseq signal ratio which emphasises the difference between Q5 and Q4 to Q1 inclusive. Q5 is consistent with hiBiv and Q4 to Q1 account for loBiv. Right panel: we performed a similar quintile division of the data underlying Fig. 6a. Q5 quintile (which is most related to hiBiv) shows most H3K27me3-sensitivity to DNA hypomethylation comparing HMEC to HCC1954/MCF7 and colon to HCT116. In contrast, Q4 to Q1 show an attenuated H3K27me3-response to DNA hypomethylation.