Additional file 11: of The transcription factor PRO44 and the histone chaperone ASF1 regulate distinct aspects of multicellular development in the filamentous fungus Sordaria macrospora

Figure S10. Expression of pks and nrps genes in different mutants and tissues. A. Heatmap was generated based on the log2 ratios from the DESeq2 analysis of RNA-seq data. Two pks genes that were previously shown to be involved in fruiting body formation (pks4 for fruiting body formation and morphology, pks7 for melanin formation for black pigmentation of perithecia and ascospores) are given in bold. The results show that pks4 is indeed upregulated in Δasf1, but not Δpro44 mycelium. Furthermore it is upregulated in all protoperithecia samples compared to wild type, again showing that expression is not strongly dependent on pro44, pro1, or nox1. Overall, several pks and nrps genes are upregulated in Δasf1 mycelia, more than in Δpro44 mycelia, and about half of the genes are strongly upregulated in wild type protoperithecia. B. Expression of pks4 in transcription factor or chromatin modifier mutants. Quantitative RT-PCR analysis of pks4 expression was performed, ratios and standard errors (given as error bars) were calculated with REST. Dots indicate significantly differential expression (REST, p < =0.05). Data for pro1 and pro44 are from Schindler and Nowrousian 2014 (Fungal Genet Biol 68: 48–59). (PDF 199 kb)