Additional file 11: of Performance evaluation of commercial library construction kits for PCR-based targeted sequencing using a unique molecular identifier

Correlation between the expected allele frequency of variants in the reference material and observed allele frequency of variants obtained using the Qiagen HASTP. Because the variants present at allele frequencies of 0.1% or 0.13% were not detected by the Qiagen data analysis center or Lofreq/Pindel, the reads supporting the reference and alternative nucleotides at the corresponding positions were counted by mpielup to calculate the observed allele frequencies. (JPG 92 kb)