Additional file 11: of M6A-mediated upregulation of LINC00958 increases lipogenesis and acts as a nanotherapeutic target in hepatocellular carcinoma

Figure S6. The LINC00958/miR-3619-5p pathway modulates lipogenesis by targeting HDGF. (A) Effects of HDGF overexpression on cholesterol level in LINC00958-silenced HCCLM3 cells. The data are shown as the mean ± SEM. ***P < 0.001 vs. the sh-NC group. (B) Effects of HDGF overexpression on triglyceride level in LINC00958-silenced HCCLM3 cells. The data are shown as the mean ± SEM. ***P < 0.001 vs. the sh-NC group. (C) RT-qPCR assays were used to examine the effects of HDGF overexpression on SREBP1 level in LINC00958-silenced HCCLM3 cells. The data are shown as the mean ± SEM. ***P < 0.001 vs. the sh-NC group. (D) Western blotting was performed to investigate the effects of HDGF overexpression on SREBP1 level in LINC00958-silenced HCCLM3 cells. (E) Oil Red O staining showing the effects of HDGF overexpression on lipid droplet formation in LINC00958-silenced HCCLM3 cells.