Additional file 11: Figure S7. of Epigenetic silencing of CREB3L1 by DNA methylation is associated with high-grade metastatic breast cancers with poor prognosis and is prevalent in triple negative breast cancers

CREB3L1 mRNA expression varies in breast tumors with CREB3L1 gene methylation in specific regions. For each of the CpG regions shown, tumor samples were divided into low and high methylation groups based on the level of methylation relative to the median methylation in that region in normal breast tissue and plotted according to the level of CREB3L1 mRNA expression for the corresponding samples. Number of samples in each group: site 2: 418-L, 133-H; site 3: 451-L, 100-H; site 16: 114-L, 437-H; site 19: 32-L, 519-H; site 20: 27-L, 524-H. The median methylation in normal breast tissue was as follows: site 2 (0.2556), site 3 (0.4189), site 16 (0.2708), site 19 (0.3352), and site 20 (0.4669). Methylation was significantly different between tumor and normal breast tissue in all regions: region 2 (p = 1.9 × 10−21), region 3 (p = 4.0 × 10−20), region 16 (p = 4.6 × 10−5), region 19 (p = 6.4 × 10−10) and region 20 (p = 1.1 × 10−7). (PDF 39 kb)