Additional file 11: Figure S3. of Thymosin beta 10 is a key regulator of tumorigenesis and metastasis and a novel serum marker in breast cancer

TMSB10 promotes the cell cycle of breast cancer cells. a and b Real-time PCR and western blot of the indicated breast cancer cells transfected with TMSB10-vector, TMSB10, TMSB10-RNAi-vector, TMSB10-RNAi#1 and TMSB10-RNAi#2. Each bar represents the mean values ± SD of three independent experiments. *P < 0.05. c Representative images of sections sliced from the indicated tumors and stained with anti-TMSB10 and anti-Ki67, respectively (left panel). Average Ki67 staining score in the indicated tissues (right panel). d Representative micrographs and quantification of BrdU incorporation in the indicated cells. Each bar represents the mean values ± SD of three independent experiments. *P < 0.05. e Real-time PCR analysis of cyclinD1, cyclinE1, CDK6, CDK4, CDKN1A and CDKN1B expression in the indicated cells. Each bar represents the mean values ± SD of three independent experiments. *P < 0.05. f Western blot analysis of cyclinD1, cyclinE1, CDK6, CDK4, CDKN1A, CDKN1B and p-Rb expression in the indicated cells. (PDF 354 kb)