Additional file 10: of Zebrafish prdm12b acts independently of nkx6.1 repression to promote eng1b expression in the neural tube p1 domain

Expression of GAL4DBD-Prdm12b constructs used in transfection experiments. a. Immunoblot showing expression of GAL4DBD-Prdm12b constructs in transfected HEK293T cells. All constructs are stable except Myc-GAL4-∆PR-prdm12b. b. Immunoblot showing expression of Myc-Flag-G9a and Myc-Flag-Bhlhe22 constructs in transfected HEK 293 T cells. (PDF 619 kb)