Additional file 10: of Renal cancer: new models and approach for personalizing therapy

Figure S7. (A) Two-way unsupervised hierarchical clustering of RPPA data of 2 G1, 7 G2, 8 G3, and 3 G4 ccRCC populations for the expression of stem cell markers reported as heatmap. (B) mRNA expression of SOX2 gene in G2, G3, and G4 ccRCC samples as assessed by RT-qPCR. Mean of three independent experiments is reported. RRN18S was used as endogenous control. *p < 0.05 (C) Representative immunofluorescence staining of tumor derived spheroids showing positivity for SOX2 (red). Merge is the over-lapping of SOX2 and DAPI staining. Confocal-microscope used Olympus, Fluoview FV1000 (Tokyo, Japan, http://www.olympus-global.com), magnification 40X. (PDF 246 kb)